All lanes : Anti-Borealin/CDCA8 antibody - N-terminal (ab171148) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with Borealin/CDCA8Lysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human esophageal carcinoma tissue labeling Borealin/CDCA8 with ab171148 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells labeling Borealin/CDCA8 with ab171148 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.