Lane 1 : Anti-BRCC36 antibody (ab62075) at 0.5 µg/mlLane 2 : Anti-BRCC36 antibody (ab62075) at 1 µg/mlLane 3 : Anti-BRCC36 antibody (ab62075) at 2 µg/mlLane 1 : MCF7 cell lysateLane 2 : MCF7 cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 10 µg per lane.Secondaryanti-rabbit IgGObserved band size : 36 kDa (why is the actual band size different from the predicted?)
ab62075 at 2.5 µg/ml staining BRCC36 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunofluorescence of BRCC36 in Human Brain cells using ab62075 at 20 ug/ml.