Anti-BRD3 antibody
| Name | Anti-BRD3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56342 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 418-556 of Human BRD3 |
| Description | Mouse Monoclonal |
| Gene | BRD3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
BRD3 antibody (ab56342) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
BRD3 antibody (ab56342) at 1ug/lane + HeLa cell lysate at 25ug/lane.
![Overlay histogram showing HeLa cells stained with ab56342 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56342, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/15480_BRD3-Primary-antibodies-ab56342-1.jpg)
Overlay histogram showing HeLa cells stained with ab56342 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56342, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.