Anti-BRD8 antibody
| Name | Anti-BRD8 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab17969 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Human, Rat, Rabbit, Horse, Guinea Pig, Dog, Pig, Monkey, Ape, Opossum, Hamster, Elephant |
| Antigen | This information is considered to be commercially sensitive |
| Description | Rabbit Polyclonal |
| Gene | BRD8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab17969 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.