Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse plasmacytoma tissue labelling BRG1 with ab70558 at 1/1000 (0.2µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling BRG1 with ab70558 at 1/1000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-BRG1 antibody (ab70558) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of Human BRG1 by Western Blot of Immunprecipitate. All lanes: ab70558 at 1µg/ml staining BRG1 in HeLa whole cell lysates immunoprecipitated using the following antibodies: Lane 1: ab70558 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.
ICC/IF image of ab70558 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70558, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.