All lanes : Anti-BRUNOL6 antibody [EPR10155] (ab173282) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal brain lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BRUNOL6 using ab173282 at a 1/50 dilution.
Flow cytometry analysis of permeabilized 293T cells labeling BRUNOL6 (red), using ab173282 at a 1/10 dilution, or negative control rabbit IgG (green)