All lanes : Anti-BSCL2 antibody (ab106793) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLane 4 : Testis (Mouse) Tissue LysateLane 5 : Testis (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-BSCL2 antibody (ab106793) at 1/1000 dilutionLane 1 : Wild type mouse white adipose tissue lysate (whole)Lane 2 : Knockout mouse white adipose tissue lysate (whole)Lysates/proteins at 50 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonaldeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of BSCL2 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106793, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.