Anti-BTK antibody (ab71765) at 1/100 dilution + Ramos cell lysate at 12.5 µg
All lanes : Anti-BTK antibody (ab71765) at 1/500 dilutionLane 1 : Non transfected HEK 293 cell lysateLane 2 : BTK transfected HEK 293 cell lysateLysates/proteins at 1 µg per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab71765 at 1/50 dilution followed by use of a peroxidase-conjugated secondary antibody and DAB staining.