BTK

Gene Symbol BTK
Entrez Gene 695
Alt Symbol AGMX1, AT, ATK, BPK, IMD1, PSCTK1, XLA
Species Human
Gene Type protein-coding
Description Bruton agammaglobulinemia tyrosine kinase
Other Description B-cell progenitor kinase|agammaglobulinaemia tyrosine kinase|dominant-negative kinase-deficient Brutons tyrosine kinase|truncated Bruton agammaglobulinemia tyrosine kinase|tyrosine-protein kinase BTK|tyrosine-protein kinase BTK isoform (lacking exon 14)
Swissprots B2RAW1 Q32ML5 Q06187
Accessions AAA61479 AAB60639 AAB64205 AAC51347 AAZ38824 EAX02856 EAX02857 EAX02858 Q06187 AF153364 AAF61477 AF153755 AAF37332 AF153756 AAF37333 AF153757 AAF37334 AF153758 AAF37335 AF153759 AAF37336 AF153760 AAF37337 AF153761 AAF37338 AF153762 AAF37339 AF153763 AAF37340 AF153764 AAF37341 AJ888376 CAI59842 AJ888377 CAI59836 AJ888378 CAI59837 AJ888379 CAI59838 AJ888380 CAI59839 AJ888381 CAI59840 AK057105 BAG51864 AK225577 AK309851 AK314382 BAG37008 AM051275 CAJ19681 AM051276 CAJ19682 AM051277 CAJ19683 AM051278 CAJ19684 AM051279 CAJ19685 AM051280 CAJ19686 AM051281 CAJ19687 AM051282 CAJ19688 AM051283 CAJ19689 AM051284 CAJ19690 AM051285 CAJ19691 AM051286 CAJ19692 AU107296 AV733045 BC109079 AAI09080 BC109080 AAI09081 DA619542 DB331677 DB636737 KF241986 AGW32074 X58957 CAA41728 NM_000061 NP_000052 NM_001287344 NP_001274273 NM_001287345 NP_001274274
Function Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. E
Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
Tissue Specificity Predominantly expressed in B-lymphocytes.
Top Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency, Osteoclast differentiation

Btk (D3H5) Rabbit mAb (Biotinylated) - 12624 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3H5
Western blot analysis of Jurkat and Ramos cell extracts using Btk (D3H5) Rabbit mAb (Biotinylated).

Btk (D3H5) Rabbit mAb (PE Conjugate) - 14192 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3H5
Flow cytometric analysis of Jurkat cells (blue) and Ramos cells (green) using Btk (D3H5) Rabbit mAb (PE Conjugate).

Phospho-Btk (Tyr223) Antibody - 5082 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM (12 µg/ml for 10 minutes), using Phospho-Btk (Tyr223) Antibody (upper) or Btk (C82B8) Rabbit mAb #3533 (lower).

Phospho-Btk (Ser180) (3D3) Mouse mAb - 3537 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3D3
Western blot analysis of extracts from hydrogen peroxide treated or serum starved THP1 cells using Phospho-Btk (Ser180) (3D3) Mouse mAb.

Btk (D3H5) Rabbit mAb - 8547 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3H5
Western blot analysis of extracts from various cell lines using Btk (D3H5) Rabbit mAb.

Btk (C82B8) Rabbit mAb - 3533 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C82B8
Western blot analysis of extracts from various cell types using Btk (C82B8) Rabbit mAb.

Phospho-Btk (Tyr223) Antibody - 3531 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-Btk (Ser180) (7A12) Mouse mAb - 3536 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 7A12

Btk Antibody - 3532 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of various cell lysates using BTK Antibody.

Btk (N-20) - sc-1108 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Btk (N-20): sc-1108. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of cells in germinal centers and cells in non-germinal centers.

Btk (M-138) - sc-1696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Btk (M-138): sc-1696. Western blot analysis of Btk expression in U-937 (A), U-698-M (B), BJAB (C) and mouse PBL (D) whole cell lysates.

Btk (10d11) - sc-81170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Btk (10d11): sc-81170. Western blot analysis of Btk expression in K-562 whole cell lysate.

Btk (7F12H4) - sc-81159 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Btk (7F12H4): sc-81159. Western blot analysis of Btk expression in K-562 (A), MCF7 (B), Jurkat (C) and HEK293 (D) whole cell lysates.

Btk (C-20) - sc-1107 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Btk (C-20): sc-1107. Western blot analysis of Btk expression in MEG-01 (A) and NAMALWA (B) whole cell lysates.

Btk (E-9) - sc-28387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Btk (E-9): sc-28387. Western blot analysis of Btk expression in MEG-01 (A), NAMALWA (B), BJAB (C) and U-698-M (D) whole cell lysates.

Btk (DFS) - sc-81735 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Btk (DFS): sc-81735. Western blot analysis of Btk expression in K-562 (A), NAMALWA (B) and U-698-M (C) whole cell lysates.

Btk (DFW) - sc-81736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Btk (DFW): sc-81736. Western blot analysis of Btk expression in Meg-01 (A), NAMALWA (B), U-937 (C), U-698-M (D), BJAB (E) and K-562 (F) whole cell lysates and mouse recombinant Btk fusion protein (G).

Anti-BTK (phospho S180) antibody [3i5] - ab14393 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3i5

Anti-BTK (phospho Y223) antibody - ab51210 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BTK (phospho Y223) antibody (ab51210)Lane 1 : Extracts from Hela cells treated with serum (10%, 15min) with no immunizing peptideLane 2 : Extracts from Hela cells treated with serum (10%, 15min) with immunizing peptide

Anti-BTK (phospho Y223) antibody [EP420Y] - ab68217 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP420Y
All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/10000 dilution (purified)Lane 1 : Untreated K562 cell lysateLane 2 : K562 cells treated with pervanadateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-BTK (phospho Y550) antibody - ab52192 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BTK (phospho Y550) antibody (ab52192) at 1/500 dilutionLane 1 : extracts from HeLa cells, treated with H2O2Lane 2 : extracts from HeLa cells, treated with H2O2 with blocking peptide

Anti-BTK (phospho Y551) antibody - ab180549 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-BTK (phospho Y551) antibody [EP267Y] - ab40770 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP267Y
All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/2000 dilutionLane 1 : K562 cell lysate non-treatedLane 2 : K562 cell lysate + PervanadateLane 3 : Jurkat cell lysate non-treatedLane 4 : Jurkat cell lysate + Pervanadate

Anti-BTK antibody - ab25971 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-BTK antibody (ab25971) at 0.5 µg/mlLane 2 : Anti-BTK antibody (ab25971) at 1 µg/mlLane 3 : Anti-BTK antibody (ab25971) at 2 µg/mlLane 1 : for analysis of U937 cell lysate (15ug lysate/lane)Lane 2 : for analysis of U937 cell lysate (15ug lysate/lane)Lane 3 : for analysis of U937 cell lysate (15ug lysate/lane)

Anti-BTK antibody - ab71765 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BTK antibody (ab71765) at 1/100 dilution + Ramos cell lysate at 12.5 µg
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