ab114015 at 1/100 dilution staining BubR1 in COS7 cells transiently transfected by pCMV6-ENTRY BubR1 and analyzed by immunofluorescence.
All lanes : Anti-BubR1 antibody [6E3] (ab114015) at 1/500 dilutionLane 1 : pCMV6-ENTRY CONTROL transfected HEK293T cell lysateLane 2 : pCMV6-ENTRY BubR1 transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.
All lanes : Anti-BubR1 antibody [6E3] (ab114015) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab114015 staining BubR1 in HEK293T cells transfected with BubR1 overexpression plasmid (red) or empty vector control (blue), and analyzed by flow cytometry.
ab114015 staining BubR1 in HeLa cells (red) compared to a non-specific negative control (blue), and analyzed by flow cytometry.
ab114015 staining BubR1 in Jurkat cells (red) compared to a non-specific negative control (blue), and analyzed by flow cytometry.