BUB1B

Gene Symbol BUB1B
Entrez Gene 701
Alt Symbol BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1
Species Human
Gene Type protein-coding
Description BUB1 mitotic checkpoint serine/threonine kinase B
Other Description MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1 homolog beta|mitotic checkpoint kinase MAD3L|mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Swissprots O60627 O60501 B4DLG3 O60566 Q8WV50 B4DL09 O75389 Q59HH6 O60758 Q96KM4 B2R6U0
Accessions AAL10712 ADF57218 EAW92394 O60566 AB208782 BAD92019 AF035933 AAC23736 AF046079 AAC12730 AF046918 AAC33435 AF053306 AAC06260 AF068760 AAC19118 AF107297 AAD11941 AI742619 AK296795 BAG59371 AK296984 BAG59525 AK312709 BAG35587 AL597325 BC018739 AAH18739 JF432304 ADZ15521 NM_001211 NP_001202
Function Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. {ECO:0000269|PubMed:10477750, ECO:0000269|PubMed:11702782, ECO:0000269|PubMed:14706340, ECO:0000269|PubMed:15020684, ECO:0000269|PubMed:19411850, ECO:0000269|PubMed:19503101}.
Subcellular Location Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
Tissue Specificity Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. {ECO:0000269|PubMed:10593653}.
Top Pathways Cell cycle, HTLV-I infection

Bub1b Antibody - 4116 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from K562 and NTERA cells using Bub1b Antibody.

Bub1b (D32E8) Rabbit mAb - 5421 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D32E8
Western blot analysis of extracts from HEK293 and HeLa cells using Bub1b (D38E8) Rabbit mAb.

BUBR1 (C-20) - sc-16195 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BUBR1 (C-20): sc-16195. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

BUBR1 (N-20) - sc-16193 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BUBR1 (N-20): sc-16193. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

BUBR1 (8G1) - sc-47744 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BUBR1 (8G1): sc-47744. Western blot analysis of BUBR1 expression in Jurkat whole cell lysate.

BUBR1 (H-23) - sc-130708 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
BUBR1 (H-23): sc-130708. Western blot analysis of BUBR1 expression in non-transfected (A) and human BUBR1 transfected (B) 293 whole cell lysates.

Anti-BubR1 antibody - ab54894 from Abcam

Prices $398.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
BubR1 antibody (ab54894) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.

Anti-BubR1 antibody - ab172581 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling BubR1 with ab172581 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - BubR1, CyclinB, DAPI, Merge.

Anti-BubR1 antibody - ab70544 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BubR1 with ab70544 at 1/200 (1µg/ml). Detection: DAB.

Anti-BubR1 antibody - ab167541 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-BubR1 antibody (ab167541) at 1/500 dilutionLane 1 : BubR1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse IgG HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-BubR1 antibody - ab70173 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BubR1 antibody (ab70173) at 1/500 dilutionLane 1 : extracts from HeLa cells treated with H2O2 (100uM, 30mins),Lane 2 : extracts from HepG2 cells treated with H2O2 (100uM, 30mins),Lane 3 : extracts from HeLa cells treated with H2O2 (100uM, 30mins), with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-BubR1 antibody - ab95146 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelled with ab95146 at 1/250 dilution followed by DAB detection. Epitope retrieval was performed.

Anti-BubR1 antibody [6E3] - ab114015 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E3
ab114015 at 1/100 dilution staining BubR1 in COS7 cells transiently transfected by pCMV6-ENTRY BubR1 and analyzed by immunofluorescence.

Anti-BubR1 antibody [8G1] - ab4637 from Abcam

Prices $400.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 8G1
Immunofluorescent imaging of an asynchronous cycling population of human cells (U2OS) with ab4637 strikingly confirms the specificity of this antibody.  No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores.  Image reveals kinetochores at prometaphase, and exactly agrees with numerous published images of BubR1 (see for example Johnson et al J Cell Sci. 117(Pt 8):1577-89 (2004). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primaryantibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C.

Anti-BubR1 antibody [EPR12259(2)] - ab183496 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12259(2)
Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/20000 dilution + HeLa lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-BubR1 antibody - N-terminal - ab135682 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BubR1 antibody - N-terminal (ab135682) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the BubR1 geneLysates/proteins at 2 µg per lane.

Anti-BUB1B / BubR1 Antibody (clone 6E3) - LS-C114772 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E3
Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.

Anti-BUB1B / BubR1 Antibody (aa1-50) - LS-C287828 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human BubR1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BubR1 used at a dilution of 1:200 (1 ug/ml).

Anti-BUB1B / BubR1 Antibody - LS-C331686 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using BUB1B antibody.

Anti-BUB1B / BubR1 Antibody (clone 2097C2a) - LS-C342733 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2097C2a
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BUB1B mAb.

Anti-BUB1B / BubR1 Antibody (aa1-50) - LS-C286717 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human BubR1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BubR1 used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-BUB1B / BubR1 Antibody IHC-plus™ - LS-B4854 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-BUB1B / BubR1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4854 concentration 10 ug/ml.

Anti-BUB1B / BubR1 Antibody (clone 3C7) - LS-C114631 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C7
IHC of paraffin-embedded Carcinoma of lung tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).

Anti-BUB1B / BubR1 Antibody (aa350-400) - LS-C287279 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-BUB1B / BubR1 Antibody (clone 6E5) - LS-C114704 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E5
IHC of paraffin-embedded pancreas tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).
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