Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/20000 dilution + HeLa lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/100000 dilutionLane 1 : HepG2 lysateLane 2 : Jurkat lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human spleen labeling BubR1 with ab183496 at 1/100 dilution and HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma labeling BubR1 with ab183496 at 1/100 dilution and HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.
Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling BubR1 with ab183496 at 1/100 dilution and Goat anti rabbit IgG (FITC). Rabbit monoclonal IgG was used as an isotype control.
Immunoprecipitation of HepG2 cells labeling BubR1 with ab183496 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.