Anti-c-Myb antibody
| Name | Anti-c-Myb antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59233 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA IHC-P |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic non-phosphopeptide derived from human c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E) |
| Description | Rabbit Polyclonal |
| Gene | MYB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Staining of C-Myb in human breast carcinoma tissue (paraffin section) with ab59233 (1/50 dilution) by Immunohistochemistry; in the absence or presence of the immunizing peptide.
ICC/IF image of ab59233 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59233, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.