Mouse LRIG1 Antibody
| Name | Mouse LRIG1 Antibody |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF3688 |
| Prices | $99.00, $365.00 |
| Sizes | 25 µg, 100 µg |
| Host | Goat |
| Isotype | IgG |
| Applications | FC ICC/IF WB |
| Species Reactivities | Mouse |
| Antigen | Mouse myeloma cell line NS0-derived recombinant mouse LRIG1 Ala37-Thr794 Accession # P70193 |
| Purity/Format | Antigen Affinity-purified |
| Description | Goat |
| Gene | LRIG1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Mouse LRIG1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109 ). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575 ) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
LRIG1 in bEnd.3 Mouse Cell Line. LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001 ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .
Detection of LRIG1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C , open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107 ).
Product References
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