Anti-C11B2 antibody [EPR10495]

• Supplier: Abcam
• Catalog: ab167413
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10495
• Applications: WB IHC-P IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to residues in Human C11B2 (UniProt: P19099)
• Description: Rabbit Monoclonal
• Gene: CYP11B2
• Conjugate: Unconjugated

Product images

Overlay histogram showing MCF7 cells stained with ab167413 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab167413, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-C11B2 antibody [EPR10495] (ab167413) at 1/1000 dilutionLane 1 : Human adrenal cell lysateLane 2 : NIH:OVCAR-3 cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C11B2 with ab167413 at 1/250 dilution.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C11B2 with ab167413 at 1/250 dilution.

Detection of C11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.