Anti-C12orf48 antibody - C-terminal
| Name | Anti-C12orf48 antibody - C-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab107308 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, between amino acids 529-558 from the C terminal region of Human C12orf48 (NP_060385 |
| Description | Rabbit Polyclonal |
| Gene | PARPBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab107308 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107308 at 5µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-C12orf48 antibody - C-terminal (ab107308) at 1/100 dilution + A549 cell line lysate at 35 µg