Detection of CXCL17/VCC-1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
CXCL17/VCC-1 in A549 Human Lung Carcinoma Cell Line. CXCL17/VCC-1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007 ) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .