Detection of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glypican 6 Monoclonal Antibody (Catalog # MAB2845, filled histogram) or isotype control antibody (Catalog # MAB002 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B ). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004 ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005 ). View our protocol for Staining Intracellular Molecules .