ZnT-8 in Human Pancreas. ZnT-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ZnT-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7318) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .