Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution + human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
All lanes : Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilutionLane 1 : C1GALT1C1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
ab68310 staining C1GALT1C1 in the Human Panc-1 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBST + 1% Donkey serum) for 1 hour at 22°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody. Key Green-C1GALT1C1, Red-ab24586 Giantin-Golgi marker, Blue-Nucleus (DAPI)See Abreview