Detection of TIGIT in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium ionomycin for 24 hours were stained with Sheep Anti-Mouse TIGIT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7267) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127 ) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P ). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A ).