TIGIT

Gene Symbol TIGIT
Entrez Gene 201633
Alt Symbol VSIG9, VSTM3, WUCAM
Species Human
Gene Type protein-coding
Description T cell immunoreceptor with Ig and ITIM domains
Other Description T-cell immunoreceptor with Ig and ITIM domains|V-set and immunoglobulin domain containing 9|V-set and immunoglobulin domain-containing protein 9|V-set and transmembrane domain containing 3|V-set and transmembrane domain-containing protein 3|Washington University cell adhesion molecule
Swissprots Q8N877 Q495A1 Q6MZS2 Q495A3 Q5JPD8
Accessions EAW79602 Q495A1 AK097192 BAC04973 AL833175 CAI46183 BC101288 AAI01289 BC101289 AAI01290 BC101290 AAI01291 BC101291 AAI01292 BX640915 CAE45956 EU675310 ACD74757 XM_011512538 XP_011510840 XM_011512539 XP_011510841 NM_173799 NP_776160
Function Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. {ECO:0000269|PubMed:19011627}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:19011627}; Single-pass type I membrane protein {ECO:0000269|PubMed:19011627}.
Tissue Specificity Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). {ECO:0000269|PubMed:19011627}.
Top Pathways Cell adhesion molecules (CAMs)

VSTM3 (A-13) - sc-103319 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-TIGIT antibody - C-terminal - ab136311 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TIGIT antibody - C-terminal (ab136311) at 1 µg/ml + HepG2 cell lysate at 10 µgSecondaryGoat anti-rabbit IgG HRPdeveloped using the ECL technique

Anti-TIGIT Antibody (aa21-234, Biotin) - LS-C297481 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TIGIT Antibody (aa28-246, Biotin) - LS-C299005 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TIGIT Antibody (aa28-246, FITC) - LS-C301486 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TIGIT Antibody (aa21-234, FITC) - LS-C303528 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TIGIT Antibody (aa21-234) - LS-C296579 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant TIGIT.

Anti-TIGIT Antibody (aa28-246) - LS-C296581 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant TIGIT.

Anti-TIGIT Antibody (aa28-227) - LS-C296584 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant TIGIT.

TIGIT Antibody (741182) [Unconjugated] - MAB7898 from Novus Biologicals

Prices $99.00, $279.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 741182
Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

TIGIT Antibody (741182) [Alexa Fluor 700] - FAB7898N from Novus Biologicals

Prices $329.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone 741182
Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human TIGIT Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7898N) or (B) Mouse IgG2B Alexa Fluor 700 Isotype Control (Catalog # IC0041N). View our protocol for Staining Membrane-associated Proteins.

TIGIT Antibody (741182) [Allophycocyanin] - FAB7898A from Novus Biologicals

Prices $359.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone 741182
Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human TIGIT APC-conjugated Monoclonal Antibody (Catalog # FAB7898A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

TIGIT Antibody (741182) [Phycoerythrin] - FAB7898P from Novus Biologicals

Prices $309.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone 741182
Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human TIGIT PE-conjugated Monoclonal Antibody (Catalog # FAB7898P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Anti-TIGIT (181-194) antibody produced in rabbit - SAB1103801 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B> <BR/>Lysate of HEK-293T cells overexpressing human TIGIT was separated on SDS-PAGE, blotted with Anti-TIGIT (181-194) (<B>Cat. No. SAB1103801</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B> <BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + TIGIT immunizing peptide (human, 181-194)

ANTI-TIGIT (C-TERM) antibody produced in rabbit - SAB1301659 from Sigma-Aldrich

Host Rabbit
Clonality Polyclonal

TIGIT Antibody - C-terminal region (ARP66485_P050) - ARP66485_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TIGIT-Antibody-C-term - AP10616b from Abgent, a WuXi AppTec company

Host Rabbit
Clonality Polyclonal
TIGIT Antibody (C-term) (Cat. #AP10616b) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the TIGIT antibody detected the TIGIT protein (arrow).

Mouse TIGIT Antibody - AF7267 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Detection of TIGIT in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium ionomycin for 24 hours were stained with Sheep Anti-Mouse TIGIT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7267) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127 ) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P ). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A ).

Mouse TIGIT Affinity Purified Polyclonal Ab, Sheep IgG - AF7267-SP from R&D Systems

Host Sheep
Clonality Polyclonal
Detection of TIGIT in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium ionomycin for 24 hours were stained with Sheep Anti-Mouse TIGIT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7267) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti‑Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Mouse TIGIT Alexa Fluor® 488-conjugated Antibody - FAB7267G from R&D Systems

Prices $329.00
Sizes 100 tests
Host Sheep
Detection of TIGIT in Mouse splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A ) and either (A) Sheep Anti-Mouse TIGIT Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7267G) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A ). View our protocol for Staining Membrane-associated Proteins .

Mouse TIGIT APC-conjugated Antibody - FAB7267A from R&D Systems

Prices $349.00
Sizes 100 tests
Host Sheep
Detection of TIGIT in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P ) and either (A) Sheep Anti-Mouse TIGIT APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7267A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A ). View our protocol for Staining Membrane-associated Proteins .

Human TIGIT APC-conjugated Antibody - FAB7898A from R&D Systems

Prices $359.00
Sizes 100 tests
Host Mouse
Clone 741182
Detection of TIGIT in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3 - cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P ) and either (A) Mouse Anti-Human TIGIT APC-conjugated Monoclonal Antibody (Catalog # FAB7898A) or (B) Mouse IgG 2B Allophycocyanin Isotype Control (Catalog # IC0041A ). View our protocol for Staining Membrane-associated Proteins .

Human TIGIT Antibody - MAB7898 from R&D Systems

Prices $99.00, $279.00
Sizes 25 µg, 100 µg
Host Mouse
Clone 741182
Detection of TIGIT in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B ) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P ). Quadrant markers were set based on control antibody staining (Catalog # MAB0041 ).

Human TIGIT MAb (Clone 741182), Mouse IgG2B - MAB7898-SP from R&D Systems

Host Mouse
Clonality Monoclonal
Clone 741182
Detection of TIGIT in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Human TIGIT PE-conjugated Antibody - FAB7898P from R&D Systems

Prices $309.00
Sizes 100 tests
Host Mouse
Clone 741182
Detection of TIGIT in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3 - cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A ) and either (A) Mouse Anti-Human TIGIT PE-conjugated Monoclonal Antibody (Catalog # FAB7898P) or (B) Mouse IgG 2B Phycoerythrin Isotype Control (Catalog # IC0041P ). View our protocol for Staining Membrane-associated Proteins .
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