Detection of Human and Rat Neuroligin 2/NLGN2 by Western Blot. Western blot shows lysates of human cortex and rat cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuroligin 2/NLGN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019 ). A specific band was detected for Neuroligin 2/ NLGN2 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .