Detection of Human and Mouse COX4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX4 Monoclonal Antibody (Catalog # MAB6980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). For addtional reference, recombinant human COX4-I1 (5 ng/lane) and recombinant human COX4-I2 (2.5 ng/lane) were included. A specific band was detected for COX4 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2 .
COX4 in NIH‑3T3 Mouse Cell Line. COX4 was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007 ) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .
COX4 in HeLa Human Cell Line. COX4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007 ) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .