Detection of Rat Draxin by Western Blot. Western blot shows lysates of rat embryonic hippocampal glial cells. PVDF Membrane was probed with 1 µg/mL of Mouse/Rat Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Draxin in Mouse Embryo. Draxin was detected in immersion fixed frozen sections of mouse embryo (E13) using Mouse Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections .