Western blot analysis of extracts of TPA treated HEL cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (upper), or AML1 Antibody #4334 (lower).
Confocal immunofluorescent analysis of HeLa cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with λ phosphatase (blue), using Phospho-AML1 (Ser249 ) Antibody compared to a nonspecific negative control antibody (red).