All lanes : Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080) at 1/1000 dilutionLane 1 : Human plasma lysateLane 2 : Human fetal liver tissue lysateLane 3 : Human fetal kidney tissue lysateLane 4 : HepG2 cell lysateLane 5 : Human milk lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Western blot analysis on immunoprecipitation pellet from fetal kidney lysate (Lane 1) or 1X PBS (negative control) (Lane 2) labeling C3 / C3a using ab171080 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 1 Primary: ab171080, 1/1000 dilutionSample: Fetal kidney tissue lysateSecondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab171080, 1/1000 dilutionSample: 1 X PBSSecondary: HRP-conjugated anti-rabbit IgG