Anti-C3 antibody
| Name | Anti-C3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab129945 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide corresponding to Human C3 aa 1600 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | C3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of C3 staining in Human normal Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab129945, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Lanes 1 & 3 : Anti-C3 antibody (ab129945) at 1 µg/mlLane 2 : Anti-C3 antibody (ab129945) at 1 µg/ml (Milk blocking - 3%)Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.