Detection of Human SPRED2 by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for SPRED2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
SPRED2 in PC-12 Rat Cell Line. SPRED2 was detected in immersion fixed PC-12 rat adrenal pheochromocytoma cell line using Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .