Detection of Mouse SLC39A4 by Western Blot. Western blot shows lysates of Hepa 1-6 mouse hepatoma cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for SLC39A4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
SLC39A4 in Mouse Small Intestine. SLC39A4 was detected in immersion fixed frozen sections of adult mouse small intestine using Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001 ) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections .
Detection of SLC39A4 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315, filled histogram) or control antibody (Catalog # AB-108-C , open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108 ).