Detection of Mouse SLC39A4 by Western Blot. Western blot shows lysates of Hepa 1‑6 mouse hepatoma cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SLC39A4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SLC39A4 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry.Hepa 1‑6 mouse hepatoma cell line was stained with Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
SLC39A4 in Mouse Small Intestine.SLC39A4 was detected in immersion fixed frozen sections of adult mouse small intestine using Goat Anti-Mouse SLC39A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7315) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.