Detection of Human and Mouse RIAM/APBB1IP by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for RIAM/APBB1IP at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
RIAM/APBB1IP in Wi-38 Human Cell Line. RIAM/APBB1IP was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010 ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .