Detection of Allergin‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human Allergin‑1 Monoclonal Antibody (Catalog # MAB7638) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Allergin‑1 in U‑87 MG Human Cell Line. Allergin‑1 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Mouse Anti-Human Allergin‑1 Monoclonal Antibody (Catalog # MAB7638) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.