Detection of DLEC/CLEC4C/BDCA‑2 in Human Whole Blood by Flow Cytometry. Human whole blood were stained with Goat Anti- Human DLEC/CLEC4C/BDCA‑2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1376) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and Human IL-3 R alpha Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB301P).Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
Detection of DLEC/CLEC4C/BDCA‑2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL‑3 R alpha/CD123 APC‑conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Goat Anti-Human DLEC/CLEC4C/BDCA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
DLEC/CLEC4C/BDCA-2 in Human Lymph Node. DLEC/CLEC4C/BDCA-2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human DLEC/CLEC4C/BDCA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.