Detection of Human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan-1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .