Detection of Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan‑1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.