Detection of Human and Mouse LRIG3 by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line, HEK293T human embryonic kidney cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LRIG3 Monoclonal Antibody (Catalog # MAB3495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for LRIG3 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
Detection of LRIG3 in KATO-III Human Cell Line by Flow Cytometry. KATO-III human gastric carcinoma cell line was stained with Mouse Anti-Human LRIG3 Monoclonal Antibody (Catalog # MAB3495, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B ).