Detection of Human Meteorin-like/ METRNL by Western Blot. Western blot shows recombinant human Meteorin-like/ METRNL (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Meteorin-like/METRNL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7867) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Meteorin-like/METRNL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .