Detection of Human Protein O-Glucosyltransferase 1/POGLUT1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protein O-Glucosyltransferase 1/POGLUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6437) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Protein O-Glucosyltransferase 1/POGLUT1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .