Detection of Human Tryptase e/BSSP-4 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tryptase e/BSSP-4 Monoclonal Antibody (Catalog # MAB5047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for Tryptase e/BSSP-4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Tryptase e/BSSP-4 in Human Pancreas. Tryptase e/BSSP-4 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Tryptase e/BSSP-4 Monoclonal Antibody (Catalog # MAB5047) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .