ab140385 stained A498 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab140385 at 1/200 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![All lanes : Anti-CA12 antibody [2C6] (ab140385) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY CA12 cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/18860_CA12-Primary-antibodies-ab140385-1.jpg)
All lanes : Anti-CA12 antibody [2C6] (ab140385) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY CA12 cDNALysates/proteins at 5 µg per lane.
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling CA12 with ab140385 at 1/150 dilution.
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling CA12 with ab140385 at 1/150 dilution.
Immunohistochemical analysis of paraffin embedded Human kidney carcinoma tissue labelling CA12 with ab140385 at 1/150 dilution.
Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling CA12 with ab140385 at 1/150 dilution.
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling CA12 with ab140385 at 1/150 dilution.