CA12

Gene Symbol CA12
Entrez Gene 771
Alt Symbol CAXII, HsT18816
Species Human
Gene Type protein-coding
Description carbonic anhydrase XII
Other Description CA-XII|carbonate dehydratase XII|carbonic anhydrase 12|carbonic dehydratase|tumor antigen HOM-RCC-3.1.3
Swissprots Q9BWG2 B2RE24 O43570 Q53YE5
Accessions EAW77646 EAW77647 EAW77648 EAW77649 EAW77650 O43570 AF037335 AAC63952 AF051882 AAC39789 AK000158 BAA90981 AK096845 BAG53376 AK315769 BAG38121 BC000278 AAH00278 BC001012 AAH01012 BC011691 AAH11691 BC023981 AAH23981 BC087838 BM994285 BQ440047 BT006656 AAP35302 BU167720 BU944741 CA309380 DA509400 DA546357 DQ893188 ABM84114 DQ896511 ABM87510 EU831616 ACE87216 EU831623 ACE87223 EU831703 ACE86537 EU831708 ACE86542 NM_001218 NP_001209 NM_001293642 NP_001280571 NM_206925 NP_996808
Function Reversible hydration of carbon dioxide.
Subcellular Location Membrane; Single-pass type I membrane protein.
Tissue Specificity Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.
Top Pathways Nitrogen metabolism

CA12 (D75C6) XP ® Rabbit mAb - 5864 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D75C6
Western blot analysis of extracts from SK-OV-3 and MCF7 cells using CA12 (D75C6) XP ® Rabbit mAb.

CA12 (D78E4) Rabbit mAb - 5865 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D78E4
Western blot analysis of extracts from SK-OV-3 and MCF7 cells using CA12 (D78E4) Rabbit mAb.

CA XII (A-3) - sc-374313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CA XII (A-3): sc-374313. Western blot analysis of CA XII expression in MCF7 whole cell lysate.

CA XII (D-2) - sc-374314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CA XII (D-2): sc-374314. Western blot analysis of CA XII expression in MCF7 whole cell lysate.

CA XII (N-20) - sc-16453 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CA XII (N-20): sc-16453. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing apical membrane and cytoplasmic staining of cells in tubules.

CA XII (H-114) - sc-25601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CA XII (H-114): sc-25601. Western blot analysis of CA XII expression in 293T whole cell lysate.

Anti-CA12 antibody - ab54917 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ICC/IF image of ab54917 stained A498 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab54917 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-CA12 antibody - ab89470 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Anti-CA12 antibody (ab89470) at 1/500 dilution + Human kidney lysate at 50 µg

Anti-CA12 antibody - ab102809 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CA12 antibody (ab102809) at 5 µg/ml + MCF7 cell lysate at 50 µgSecondaryGoat anti-Mouse IgG HRP at 1/2500 dilution

Anti-CA12 antibody [2C6] - ab140385 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C6
ab140385 stained A498 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab140385 at 1/200 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-CA12 antibody [EPR14861] - C-terminal - ab195233 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14861
Flow cytometric analysis of SK-OV-3 cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa25-291) - LS-C35272 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (clone 4G3) - LS-C337928 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4G3
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-CA12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa224-273) - LS-C291745 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa25-302, Biotin) - LS-C300911 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa154-307, Biotin) - LS-C297193 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa161-318, Biotin) - LS-C301261 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (clone 3F1) - LS-C174423 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA12.

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa231-280) - LS-C119809 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from MCF-7 cells, using CA12 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa161-318, FITC) - LS-C303958 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa154-307, FITC) - LS-C304624 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa25-302, FITC) - LS-C304675 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (aa161-318) - LS-C293157 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant CA12 / Carbonic Andhydrase XII.

Anti-CA12 / Carbonic Andhydrase XII Antibody - LS-C309669 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CA12 / Carbonic Andhydrase XII Antibody (clone 2E9) - LS-C174411 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E9
Anti-CA12 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CA12.
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