Anti-CA12 antibody [EPR14861] - C-terminal

• Supplier: Abcam
• Catalog: ab195233
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14861
• Applications: FC IHC-P WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CA12 aa 300 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: CA12
• Conjugate: Unconjugated

Product images

Flow cytometric analysis of SK-OV-3 cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cell membrane and cytoplasm staining on human stomach tissue was observed.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cell membrane and cytoplasm staining on human colon tissue was observed.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100  dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.

Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.

All lanes : Anti-CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : SKOV3 cell lysateLane 3 : 293 cell lysateLane 4 : HeLa cell lysateLane 5 : SKOV3 cell lysateLane 6 : Human fetal kidney lysateLane 7 : Human ovary cancer lysateLane 8 : Human breast cancer lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique