Detection of Human WTX by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
WTX in BG01V Human Embryonic Stem Cells. WTX was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007 ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .