Detection of TIGIT in Mouse splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A ) and either (A) Sheep Anti-Mouse TIGIT Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7267G) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A ). View our protocol for Staining Membrane-associated Proteins .