All lanes : Anti-Calcium Sensing Receptor antibody (ab18200) at 1 µg/mlLane 1 : CASR 293T Cell Transient Overexpression Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.Observed band size : 130 kDa (why is the actual band size different from the predicted?)Additional bands at : 200 kDa,50 kDa,80 kDa. We are unsure as to the identity of these extra bands.Exposure time : 3 minutesIn this experiment Hek293 was used as a negative control.
This image is courtesy of an Abreview submitted by Dr sriharsha kantamneniLane 1 = HEK cell lysate used as negative control. Lane 2 = Post nuclear fraction of rat brain (P21). As secondary goat anti-rabbit HRP conjugated was used. Please see accompanying abreview for additional informationSee Abreview