Anti-CALCOCO2 antibody
| Name | Anti-CALCOCO2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68588 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Human, Chimpanzee, Monkey, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CALCOCO2 |
| Description | Rabbit Polyclonal |
| Gene | CALCOCO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab68588 stained HeLa cells. The cells were 4% PFA fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68588 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-CALCOCO2 antibody (ab68588) at 1 µg/mlLane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell LysateLane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of CALCOCO2 staining in Human Cerebral Cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68588, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Product References
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p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different - p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different
Mostowy S, Sancho-Shimizu V, Hamon MA, Simeone R, Brosch R, Johansen T, Cossart P. J Biol Chem. 2011 Jul 29;286(30):26987-95.