CALCOCO2

Gene Symbol CALCOCO2
Entrez Gene 10241
Alt Symbol NDP52
Species Human
Gene Type protein-coding
Description calcium binding and coiled-coil domain 2
Other Description antigen nuclear dot 52 kDa protein|calcium-binding and coiled-coil domain-containing protein 2|nuclear domain 10 protein 52|nuclear domain 10 protein NDP52|nuclear dot protein 52
Swissprots E9PBE5 Q13137 Q6IBN9 B4DDT4 B4E0C0 Q53HB5 Q53FQ5 B4DP36 B2RBT0 E7ENK0 Q9BTF7 E7ETP5 B4DDC4
Accessions EAW94707 EAW94708 Q13137 AF085965 AK222666 BAD96386 AK223227 BAD96947 AK293137 BAG56685 AK293329 BAG56845 AK298177 BAG60448 AK303313 BAG64382 AK304024 BAG64939 AK310648 AK314796 BAG37327 AL042638 AL046722 BC004130 AAH04130 BC015893 AAH15893 BE315542 BG773301 BU620013 CR456763 CAG33044 HQ448259 ADQ32739 JF432510 ADZ15727 U22897 AAA75297 XM_011524181 XP_011522483 NM_001261390 NP_001248319 NM_001261391 NP_001248320 NM_001261393 NP_001248322 NM_001261395 NP_001248324 NM_005831 NP_005822
Function May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion. {ECO:0000269|PubMed:17635994}.
Subcellular Location Cytoplasm, perinuclear region. Golgi apparatus. Cytoplasm, cytoskeleton. Note=According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot- associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.
Tissue Specificity Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain. {ECO:0000269|PubMed:7540613}.

NDP52 Antibody - 9036 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human NDP52 (hNDP52; +), using NDP52 Antibody.

CALCOCO2 (C-9) - sc-393063 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALCOCO2 (C-9): sc-393063. Western blot analysis of CALCOCO2 expression in non-transfected 293: sc-110760 (A), human CALCOCO2 transfected 293: sc-112224 (B) and HeLa (C) whole cell lysates and Jurkat (D) and Ramos (E) nuclear extracts.

CALCOCO2 (E-10) - sc-393042 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALCOCO2 (E-10): sc-393042. Western blot analysis of CALCOCO2 expression in non-transfected 293: sc-110760 (A), human CALCOCO2 transfected 293: sc-112224 (B) and HeLa (E) whole cell lysates and Jurkat (C) and Ramos (D) nuclear extracts.

CALCOCO2 (F-6) - sc-376540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALCOCO2 (F-6): sc-376540. Western blot analysis of CALCOCO2 expression in Ramos nuclear extract.

CALCOCO2 (C-12) - sc-160994 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CALCOCO2 (C-12): sc-160994. Western blot analysis of CALCOCO2 expression in HeLa whole cell lysates (A) and Jurkat (B) and Ramos (C) nuclear extracts.

CALCOCO2 (N-12) - sc-160995 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CALCOCO2 (N-12): sc-160995. Western blot analysis of CALCOCO2 expression in non-transfected: sc-110760 (A) and human CALCOCO2 transfected: sc-112224 (B) 293 whole cell lysates.

Anti-CALCOCO2 antibody - ab68588 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab68588 stained HeLa cells. The cells were 4% PFA fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68588 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was  used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-CALCOCO2 antibody - ab151256 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CALCOCO2 antibody (ab151256) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-CALCOCO2 antibody - ab56665 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56665 CALCOCO2 antibody at 1ug/ml.

Anti-CALCOCO2 antibody [2623C2_2a] - ab84862 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2623C2_2a
Anti-CALCOCO2 antibody [2623C2_2a] (ab84862) + Immunogen (Recombinant Human CALCOCO2 fragment)

Anti-CALCOCO2 antibody [4H5] - ab124372 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4H5
All lanes : Anti-CALCOCO2 antibody [4H5] (ab124372) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CALCOCO2Lysates/proteins at 5 µg per lane.

Anti-CALCOCO2 antibody [6E8] - ab118054 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E8
All lanes : Anti-CALCOCO2 antibody [6E8] (ab118054) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CALCOCO2 cDNALysates/proteins at 5 µg per lane.

Anti-CALCOCO2 antibody [GT422] - ab184688 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT422
All lanes : Anti-CALCOCO2 antibody [GT422] (ab184688) at 1/1000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : Raji whole cell lysateLane 3 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-CALCOCO2 Antibody (clone 4H5) - LS-C172434 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4H5
Anti-CALCOCO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2.

Anti-CALCOCO2 Antibody - LS-C125235 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CALCOCO2 Antibody (clone 2623C2_2a) - LS-C184743 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2623C2_2a

Anti-CALCOCO2 Antibody - LS-C307755 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-CALCOCO2 Antibody (aa347-446) - LS-C307767 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CALCOCO2 Antibody - LS-C309691 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CALCOCO2 Antibody - LS-C309693 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CALCOCO2 Antibody (C-Terminus) - LS-C345313 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of CALCOCO2 Antibody with human Fetal Liver lysate.

Anti-CALCOCO2 Antibody (clone 2623C2_2a) - LS-C342772 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2623C2_2a
Western blot analysis of immunized recombinant protein, using anti-CALCOCO2 monoclonal antibody.

Anti-CALCOCO2 Antibody (clone 3F2) - LS-C172465 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F2
Anti-CALCOCO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2.

Anti-CALCOCO2 Antibody (clone 6E8) - LS-C172496 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E8
Anti-CALCOCO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2.

Anti-CALCOCO2 Antibody (clone 2B11) - LS-C337158 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B11
Anti-CALCOCO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2.
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