Anti-CALCOCO2 antibody (ab151256) at 1/1000 dilution + Raji whole cell lysate at 30 µg
All lanes : Anti-CALCOCO2 antibody (ab151256) at 1/1500 dilutionLane 1 : Huh7 whole cell lysate/extract (untreated) Lane 2 : Huh7 whole cell lysate/extract (3µM-Thapsipargin treatment for 12hr)Lysates/proteins at 20 µg per lane.
Immunohistochemical analysis of paraffin embedded H441 xenograft labeling CALCOCO2 with ab151256 antibody at 1/100.
Immunofluorescent analysis of methanol-fixed A431 cells labeling CALCOCO2 with ab151256 at 1/200. Picture at the bottom costained with Hoechst 33342.