All lanes : Anti-CALCOCO2 antibody [4H5] (ab124372) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CALCOCO2Lysates/proteins at 5 µg per lane.
All lanes : Anti-CALCOCO2 antibody [4H5] (ab124372) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2 using ab124372 at a dilution of 1/100.
HEK293T cells transfected with either CALCOCO2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124372 at a dilution of 1/100, and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using ab124372 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometric Analysis of Jurkat cells, using ab124372 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).